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Published on Aug 16, 2012
For more info, visit http://www.bio-rad.com//qPCR. This Biotechnology Explorer™ webinar presents an overview of what real-time PCR is, what it is used for, and how it works. Also known as quantitative PCR (qPCR), this technique exploits the power of exponential DNA amplification by the polymerase chain reaction coupled with fluorescent detection of the products in real time, enabling researchers to detect and measure the initial number of copies of a target DNA sequence with high sensitivity and accuracy.
Dr David Palmer, a Bay Area biotech entrepreneur and adjunct professor at Contra Costa College, explains the principles of qPCR with examples suitable for classroom instruction. Dr Palmer discusses the usefulness of this technology, citing real-world applications: measuring the expression of a cancer-related gene, determining the viral load in HIV infection, detecting GMO DNA in food, and identifying suspects at a crime scene from a single drop of blood. He shares his classroom teaching experience, relating a unique way to illustrate the calculation of starting DNA quantities from the curves of fluorescence intensity versus time.
Presenter: David Palmer, PhD Senior Technical Support Consultant Bio-Rad Laboratories
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