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Published on Feb 19, 2014
Table of Contents: 00:09 Lecture 5.2: Putting the pieces together Genome Sequencer -- Part 2 01:44 Outline 02:04 Selectivity and Sequencing 04:05 Outline 04:29 Idealized Polymerase Chain Reaction (PCR) 05:46 How PCR Doubles DNA Count 07:33 Emulsion PCR (Reall biobarcode approach) 10:27 Multiply Loaded Beads must be avoided (Control of bead to DNA ratio) 12:25 Outline 12:27 How to avoid multiple loading 16:06 Signal and Reference Well 18:06 Loading with Reference Wells
This course provides an in-depth analysis of the origin of the extra-ordinary sensitivity, fundamental limits, and operating principles of modern nanobiosensors. The primary focus is the physics of biomolecule detection in terms of three elementary concepts: response time, sensitivity, and selectivity. And, it potentiometric, amperometric, and cantilever-based mass sensors to illustrate the application of these concepts to specific sensor technologies.