nanoHUB-U Nanobiosensors L5.2: Putting the pieces together - Genome Sequencer II





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Published on Feb 19, 2014

Table of Contents:
00:09 Lecture 5.2: Putting the pieces together Genome Sequencer -- Part 2
01:44 Outline
02:04 Selectivity and Sequencing
04:05 Outline
04:29 Idealized Polymerase Chain Reaction (PCR)
05:46 How PCR Doubles DNA Count
07:33 Emulsion PCR (Reall biobarcode approach)
10:27 Multiply Loaded Beads must be avoided (Control of bead to DNA ratio)
12:25 Outline
12:27 How to avoid multiple loading
16:06 Signal and Reference Well
18:06 Loading with Reference Wells

This video is part of the nanoHUB-U course "Principles of Electronic Nanobiosensors". (https://nanohub.org/courses/PEN)

This course provides an in-depth analysis of the origin of the extra-ordinary sensitivity, fundamental limits, and operating principles of modern nanobiosensors. The primary focus is the physics of biomolecule detection in terms of three elementary concepts: response time, sensitivity, and selectivity. And, it potentiometric, amperometric, and cantilever-based mass sensors to illustrate the application of these concepts to specific sensor technologies.

For more details see http://nanohub.org/u


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