 This study has successfully established and characterized a continuous Atlantic mackerel, scombus scombris, skeletal muscle cell line, mac cells, which can be used as a reference point for future investigations into the cell's properties and characteristics. The cells have been shown to exhibit both muscle and adipose tissue characteristics, such as muscle stemness and differentiation, and have been genetically characterized using new QPCR primers tailored to the mackerel genome. This article was authored by Michael K. Saad, John S.K. Ewan, Conor M. Joyce, and others.