 This paper demonstrates the use of a specific mutation RT-PCR-based approach to identify variants of concern, VOC, in SARS-CoV-2 positive samples through the pandemic in Pakistan. The authors found that three pandemic waves associated with alpha, delta, and omicron variants occurred during the study period. They also showed that the CT values of alpha, beta, gamma, and delta were all significantly higher than that of omicron variants, P less than 0.0001. This suggests that these variants are more pathogenic and may be associated with increased virulence and severity of disease. Furthermore, this study highlights the importance of identifying VOC in order to better understand the evolutionary dynamics of the virus and inform public health responses. This article was authored by Iskrenasir, Osmobashir Amir, Auk Barkanji, and others.