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Nano-optics methods for studying DNA-protein interactions at the single-molecule level

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Published on Apr 1, 2012

Prof. Yuval Garini's presentation at Nano-Israel 2012 conference.

Abstract:
We developed a method for studying DNA-protein interactions on a single-molecule level using an optical method that is based on tethered particle motion (TPM) with a gold nano-bead. The plasmonic properties of gold nano-beads tremendously extend the measurements capabilities and allows for improved spatial and temporal measurements. Using the method we measured the three dimensional end-to-end distribution of a DNA tethered to a wall. Although the lateral distribution is well known and studied, the axial distribution was never measured before.
Finally, we used the system to measure the interaction of HU protein with DNA and confirmed a bi-modal that depends on the protein concentration. The results confirm the capabilities of rather simple optical methods to detect fundamental biological interactions at the nano-level by using nano-particles and devices.

links:
lab website: http://faculty.biu.ac.il/~gariniy
relevant papers:
http://onlinelibrary.wiley.com/doi/10...
http://pre.aps.org/abstract/PRE/v83/i...
http://www.benthamscience.com/open/to...
http://www.sciencedirect.com/science?...

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