 Hi, I'm Yu-Chien Hu. I'm a professor from the department of chemical engineering, National Tsinghua University in Taiwan. And as you know, microRNAs are small RNAs that can regulate gene expression. And the microRNAs are involved in the number of biological processes like development, cellular differentiation, oncogenesis, viral pathogenesis, and the immune responses. So microRNAs have become an attractive therapeutic agent. However, effective microRNA delivery and processing with minimal side effects remains a hurdle to its applications. Although there are a number of gene delivery vectors available, such as plasmid, adenovirus, adenosociative virus, lentivirus, and retroviruses, and so on, these vectors have their respective drawbacks. In contrast, vector virus is an insect virus. And in nature, vector virus only infects insect cells. But vector virus can also deliver foreign genes into various mammalian cells very effectively. As opposed to other viral vectors, vector virus has a number of distinct advantages. First, vector virus does not cause apparent cytotoxicity in the mammalian cells. And the vector virus does not replicate inside the mammalian cells. Thus reducing the possible side effects. Second, vector virus genome is very large. So the cloning capacity can be as large as 30 AKB. Third, recombinant vector viruses can be easily constructed and propagated to high titers by simply infecting the natural host insect cells. And finally, and very importantly, vector virus is non-pathogenic to humans. So, vector virus can be handled in biosafety level one facilities. So over the past decade, vector virus has been widely used for in vivo and in vitro gene delivery studies. Given the potential of microRNA for gene revelation and the promise of vector virus for gene delivery, in this study, we developed recombinant vector viruses carrying artificial gene specific microRNA sequences within the microRNA 155 scaffold, which after expression by the cytomeglovirus promoter could knock down the gene expression in a sequence and those dependent manner. And also by swapping the mature microRNA sequences, the baclovirus microRNA shuttle can repress the overexpression of endogenous TNF alpha by the orthotic synovial sites without inducing apoptosis. To prolong the suppressive effects, we further developed a hybrid baclovirus that exploited the sleeping beauty transposon for gene integration and sustained microRNA expression. The hybrid baclovirus vector effectively repressed the chance gene expression for a prolonged period of time, so baclovirus can be a novel vector for artificial microRNA delivery and sustained gene suppression. If you are interested in this study, please visit the biotech and bio-edge website for more details. Thank you.