 The study found that ubiquitin-mediated protein degradation plays a crucial role in various cellular processes, including signal transduction, cell differentiation, and stress response. Ubiquitin C-terminal hydrolase 1, UCHL1, is a unique tuberquitinating enzyme that has both hydrolase and ligase activities. The aim of the study was to determine the concentration of UCHL1 in serum of children with appendicitis, before and after surgery. The results showed that the UCHL1 concentrations in the blood plasma of patients with acute appendicitis were highest before the surgery and were above the range of concentrations measured in controls. The UCHL1 concentration slowly decreased over time, but still did not reach the normal range when compared with the concentration measured in controls. The method of operation, classic open appendectomy or laparoscopic appendectomy, did not influence the general trend in UCHL1 concentration in children with appendicitis. There was a strong negative correlation between pre-albumin and UCHL1 concentrations. This article was authored by Uama Tushchak, Mozena Tilika, Wojcik Debek, and others. We are article.tv, links in the description below.