 The study evaluated the impact of sucrose concentration and osmotic dehydration duration on chrysanthemum cryopreservation using encapsulation dehydration technique. The best regrowth of cryopreserved shot tips was achieved with 0.25 m sucrose concentration in 10 mABA during a two-week pre-culture followed by a four or seven-day osmotic dehydration. Higher sucrose concentration during the pre-culture slowed growth, increased formation of multiple shoots or deformed adventitious shoots, and decreased rhizogenesis efficiency. The frequency of tissue hyperhydricity also increased. Osmotic dehydration duration had a cultivar-dependent influence on explants morphogenetic response. This article was authored by Daryush Koulous, Magdalena Soraka, and Anna Makula.