 Raman spectroscopy has been identified as a potential tool for identifying traces of body fluids in forensic applications. Two techniques have been developed to address the limitations of this technology, including reducing a spectrum complexity, RSC, and multivariate curve resolution combined with addition methods, MCID. Both techniques have been tested on various substrates commonly encountered in forensic such as paper, plastic, glass, and fabric. The RSC technique has shown promise in reducing the complexity of the spectral data, while MCID has demonstrated its effectiveness in detecting blood stains on these materials. A hierarchical approach has also been proposed to further reduce the risk of false positives. This article was authored by Yuri V. Kistenev, Alexei V. Boyzov, Alisa A. Samarinova, and others.