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Chimerism Longitudinal Monitoring

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Published on Jul 12, 2011

ChimerMarker™, automated chimerism analysis software, integrates speed and accuracy with a biologist-friendly interface. The software can be used to monitor chimerism level in both allogeneic and autologous stem cells transplant (SCT) or hematopoietic stem cells transplant (HSCT), bone marrow transplant (BMT, post bone marrow engraftment), and cord and peripheral blood stem cells transplant (PBSCT) samples. The program provides accurate, rapid genotyping and chimerism analysis; automatically identifies donor and recipient peaks in post-BMT samples, calculates percent chimerism and quality metrics for single donor or double donor cases, easily appends for longitudinal monitoring post-BMT, and has multi-lineage capabilities for chimerism analysis of T-cells, B-cells, and other cell type populations. ChimerMarker includes functions for comparison of samples at different time points to conduct longitudinal studies for monitoring each individual and a comprehensive chimerism analysis report. The chimerism analysis performs repetitive calculations (using published methods. Dr. Don Kristt). ChimerMarker is compatible with ABI®PRISM, Beckman-CEQ™, and MegaBACE® genetic analyzers, and custom primers or commercially available human identification chemistries for STR genotyping (including Identiflier®, PowerPlex®16, PowerPlex®ESI). Chimerism analysis is completely linked to the main analysis screen, removing the error-prone step of data transfer from genotyping software to chimerism analysis software.

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