 Superhydrophobic surfaces have been shown to reduce bacterial attachment by creating a physical barrier between the bacteria and the surface. In this study, the effectiveness of different pillar spacings was evaluated to determine the best configuration for maximum antifouling performance. It was found that a pillar spacing of 87.5 microns provided the greatest reduction in bacterial attachment with a three-log reduction greater than 99.9% reduction. This research provides valuable insights into the complexities of superhydrophobic fouling and demonstrates how optimized pillar spacing can maximize the antifouling performance of these materials. This article is authored by Emma Sadler, Abish S. Stephen, Robert P. Allaker, and others.