 This study developed a low-cost and stable electrochemical apto-sensor for the detection of alpha-fetaprotein, AFP, in human serum. The sensor utilized vertically-ordered mesoporosilica films, VMSF, which had selenol groups on their surfaces and regularly-ordered nanopores, providing binding sites for further functionalization of recognition aptomers and conferring the sensor with excellent anti-biofiling properties. The sensor's sensitivity and specificity were enhanced by the target AFP-controlled diffusion of Fe, CN, 63-slash-4-redox electrochemical probes through the nanochannels of VMSF. This resulted in a linear relationship between the sensor's electrical signal and the AFP concentration, allowing for accurate and reliable measurements of AFP levels in human serum samples. This article was authored by Tongtong Zhang, Luo Xiangyang, Fe Yan, and others.