 Macrophages play a critical role in inflammation, a defense mechanism of the innate immune system. They rely on metabolic processes powered by glucose transporters, such as GLUT1, to carry out their functions. Inflammation can be triggered by lipopolysaccharide, LPS. This study investigates how cystathionine gamma-lyase, CSE, and its by-product, hydrogen sulfide, H2S, affect the metabolic process of macrophages during this inflammatory response. It was found that LPS increases GLUT1 expression, which is regulated through nuclear factor, NF, Kappa B activation and phosphatidilinositol-3 kinase, PI3K, activation. Silencing CSE decreases NF Kappa B activation and GLUT1 expression, indicating that H2S plays a role in metabolic function in macrophages during pro-inflammatory response. Additionally, it was discovered that GYY4137, an H2S donor molecule, has inhibitory effects on LPS-induced NF Kappa B activation and GL. This article was authored by Alex Cornwell, Samantha Fetitova, Sarah Cowan, and others. We are article.tv. Links in the description below.