 The CTS Personas Project aimed to develop 12 evidence-based profiles of roles across the translational workforce and two patients. These profiles were designed to illustrate the key responsibilities, motivations, goals, software use, pain points and professional development needs of individuals working in various areas of translational research such as basic science, clinical research and public health. The project also sought to identify potential challenges and successes associated with creating these profiles. The resulting persona profiles can be used to inform software development projects, educational resources and communication initiatives. Additionally, this paper provides insight into the process of developing persona profiles, including the methodology, engagement strategy and lessons learned. This article was offered by Sara Gonzalez, Lisa O'Keefe, Karen Gutsman and others.