 The present study evaluated the genetic diversity of 49 accessions of egelops and triticum genera in Iran using two DNA-based markers, SSR and ISSR. The results indicated that the genetic diversity within the species was greater than the genetic diversity between them. Additionally, the accessions were divided into eight clusters according to the SSR and ISSR markers. Furthermore, the genetic structure analysis successfully separated accessions for their ploidy levels. This article was offered by Murray and Jabori, Ahmed Reza Golpaver, Bezotso Kalalalu and others.