 we have developed a novel automated and quantitative high-content synaptic phenotyping platform to study the effects of small molecules on human neuronal cells. We found that certain small molecules increase the number of synapses in human neurons, suggesting that they could potentially be used to treat neurological disorders. Additionally, we identified a group of compounds that activate specific genetic pathways involved in synapse formation. This research provides a powerful tool for studying the molecular and cellular mechanisms behind synaptic development and opens the door for new therapeutic approaches for treating neurological diseases. This article was authored by Martin H. Berrier, Gizemriski, Anne Nathanson, and others.