 This study assessed the knowledge and attitudes of healthcare workers towards monkeypox in southwestern Saudi Arabia. It was found that the majority of participants had low knowledge levels and high positive attitudes towards monkeypox prevention and treatment. These findings suggest that more efforts are needed to ensure that healthcare workers are adequately trained and informed about monkeypox prevention and management. This article was authored by Nasser H. Sobeki, Najem Z. Alsharani, Rakan S. Hazatzi and others.