 Decising and scouring of cotton fabric were performed with conventional chemical and enzymatic methods, followed by dyeing with acacia-cattitude using three enzymes, acid cellulase, neutral cellulase, and xylonease, to enhance dyeing and functional properties. The phytochemical analysis revealed the presence of saponins, flavonoids, glycosides, phenols, and terpenoids in the dye. Enzyme-pre-treated fabrics had better color uptake than chemically-pre-treated fabrics, and the dyed fabrics exhibited good fastness properties, excellent UPF, and antimicrobial properties against gram plus VE and gram-VE bacteria. The xylence enzyme performed superiorly among the three enzymes. This article was authored by later sommant, Sikko Hosei, Nilam M. Rose, and others.