 Microneedle-based systems have been developed to access interstitial fluid, ISF, which is a rich source of biomarkers. These systems have the potential to revolutionize the health care industry by providing point-of-care, POC, micro-devices that can detect or monitor biomarkers in real time. Recent studies have demonstrated the feasibility of microneedle-based ISF sampling and sensing, including extraction methods, sampling volumes, sampling durations, integration with biosensors, and subsequent analysis of ISF. Opportunities, conclusions, and future prospects for developing new research in this area are also discussed. This article was authored by Khaled Muhammad Saifullah and Zarafarajirad.