 Enzyme-based biosensors are increasingly used in various applications due to their high sensitivity and specificity, portability, cost, effectiveness, and potential for miniaturization and point of care diagnostics. This review paper examines the principles behind these biosensors, which employ electrochemical, optical, thermistor, and piezoelectric detection methods and their applications in the literature. Additionally, strategies for improving the use of enzymes in biosensors are discussed. This article was authored by Hong Hiepeng-Win, Sun Hyak Lee, Uai Ching Lee, and others.