 Using a sterile puppet tip, our toothpicks let a single colony grow on solid culture media. Drop the tip or toothpick into the liquid culture media. Loosely close the cap of the glass tube so that it is not airtight. Make sure to label the details of the sample on the tube before culturing. Also make sure to have a separate tube of liquid media without any bacterial sample which would be labeled as negative control. Use liquid bacterial culture at 37 degrees Celsius for 12 to 18 hours in a shaking incubator. After incubation check for growth which is characterized by a cloudy haze in the media as shown here. Inoculating using disposable loculating loop. Using a sterile disposable loculating loop, let a single colony grow from solid culture media. Put the tube containing liquid media and put the tip of the loop inside and gently spin the loop in the media for 15 to 25 seconds as being shown. Close the cap of the glass tube so that it is not airtight. Place the samples in the incubator at 37 degrees Celsius and securely close the incubator. After 12 to 24 hours check for bacterial growth. Tubes with white haze in the liquid media shows positive growth and tubes with clear media shows no growth as compared with the negative control.