 Herpsex-DET is a comprehensive database containing information about sex determination in amphibians and reptiles. It includes data on genetically determined sex determination, temperature dependent sex determination, and cases of sex reversal. The database contains information on 192 amphibian and 697 reptilian species, making it a valuable resource for comparative studies on the evolution of sex determination and its consequences for species-specific traits like life history and conservation status. This article was authored by Idina Nemeshazi and Veronica Bocconi.