 Hi, my name is Emilia Biffy and I'm a postdoc at Politecnica di Milano in the Bioengineering Department. This is one of my co-authors, Julia Regalia, a PhD student. In our current publication on biotechnology and bioengineering, we describe the development of an environmental chamber which grows neuronal cultures on mercury to the rays and measures their electrical activity at the same time. This is a schematic representation of the whole system. A heating bath guarantees the temperature control inside the environmental chamber, while a CO2 gas cylinder and a UV refining module maintain a physiological pH and a similarity. The chamber has a characteristic dimension of 20 cm and it is made of poly-methymethacrylate, which assures portability and optical transparency. Furthermore, it is tightly closed to maintain stability and it is provided with openings for medium and drug delivery. Finally, the electronic 460 channel recording is embedded. At the moment, we use external commercial amplifiers and filters. This is the system realized. The environmental chamber is connected to the heating bath and to the gas cylinder. The chamber houses neuronal cultures on mercury to the rays and these are the boards for signal acquisition. We currently used a commercial multi-channel system for data processing and visualization, but the development of a custom electronic board is ongoing. This system was tested in terms of temperature profile, gas flow rate and medium osmolarity and its performances were compared to the standard incubator and the commercial recording system. The environmental chamber assures a temperature control at 37°C in red, comparable to a standard incubator in green and to a commercial recording system in blue. pH value was maintained constant around 7.4 as in the incubator, while the recording system showed worse performances. Finally, the chamber controlled the increase in osmolarity, which was lower than 15 miliosmol per day. Its performance was comparable to the incubator and better than the commercial recording system. Two glass cover slips and standard 60-electrode Mia biochips were used as substrate for cell plating. Primary neuronal cultures were obtained from CD1 mice at E17.5. Hippocampi were extracted, rinsed with HBSS and treated with trypsin. Then, cells were resuspended in the plating medium and mechanically associated using glass pipettes. Finally, cells were plated at 200 cells per millimeter square on cover slips and 800 cells per millimeter square on meas. Finally, cells were incubated at 37°C. Glass cover slips were incubated for 21 days and the neuronal viability was measured with dead-life staining. Salviability was comparable to standard incubators. Then, the activity of neurons on meas was measured and the signal features were comparable to the commercial recording system. The great advantage of the system is that it improves long-term stability of a neuronal signal and thus allows long-term investigations. Thank you and now enjoy the reading.