 polypyrimidine-tracked binding proteins, PTBPs, important regulators of alternative splicing in neurons. PTBP1 is widely expressed throughout the body while PTBP2 is primarily found in neurons. This study used brain tissues and human-induced pluripotent stem cells, IPSCs, derived from neurons to investigate how PTBP2 affects alternative splicing. The researchers identified PTBP2 binding sites on RNA molecules and studied their effects on alternative splicing. They also identified new PTBP2 targets, such as SYNGAP1, which is involved in synapse formation and function. Finally, they developed antisense oligonucleotide ASO, therapy to target PTBP2 and restore SYNGAP1 levels in patient-derived IPSC neurons. Their results provide insight into the mechanisms behind alternative splicing in neurons and suggest potential treatments for neurological diseases caused by defective splicing. This article was authored by Jenine M. DeWicka McKenna, Alex J. Felix, Eliza A. Waxman, and others. We are article.tv, links in the description below.