 Isolation and handling of microbial flora of fermented product aspergillus. Introduction Genus aspergillus mainly consists of different mould species that differ according to their availability in different climatic conditions. Most common fungus is aspergillus niger, which is basic contaminant of food. It causes diseases which is known as black mould disease mostly occurs in fruits and vegetables such as apricots, onions, etc. For isolation and handling of aspergillus, we used pearl millet as a fermented product for the preparation of pearl millet falling procedure is used. For preparation of pearl millet, purchase pearl millet from market in a sterile bottle washed with the help of distilled water and then dry under sun. After drying, prepare powdered pearl millet with the help of sterile blender. This is sterile distilled water of powdered pearl milk distilled water. Sit properly incubator for 7 days for fermentation. To fermentation dilution from fermented pearl millet for cereal dilution form fermented pearl millet solution and dilute it to 5 cereal dilutions. Coolation extract agar plate is prepared to minus 4 dilution. Inoculum onto the mild extract agar plate tightly over the surface of agar. Inoculum is fully absorbed on the surface of agar. Place the plate in incubator for 2-4 days for isolation of aspergillus nigel.