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Published on Jun 26, 2012
For more info, visit http://www.bio-rad.com/yt/6/Rotofor. This video tutorial demonstrates sample loading and operation of Bio-Rad's Rotofor cell for a focusing run, starting with a previously assembled focusing chamber and electrodes. The narrator also shows how to remove air bubbles from the chamber and set the power supply. The Rotofor cell offers rapid, simple, and effective fractionation and enrichment of proteins from complex protein mixtures.
Due to the great complexity of samples such as tissues, cells, and body fluids, protein researchers may want to fractionate crude samples prior to analysis by two-dimensional (2-D) gel electrophoresis. Fractionation is particularly relevant when trying to detect low-abundance proteins in samples like serum, cerebrospinal fluid (CSF), and urine because of the presence of highly abundant protein such as albumin and immunoglobulins. The Rotofor and mini Rotofor cells use liquid-phase IEF to fractionate small-medium volumes (3-60 ml) of proteins in free solution based on isoelectric point (pI). Protein fractionation by pI improves sample loading and separation on narrow- and micro-range IPG strips in downstream 2-D electrophoresis applications.
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