 Acute respiratory distress syndrome, ARDS, is a global health issue. Its pathophysiology involves an immune response in the lungs, leading to lung inflammation and damage. To address this problem, a rapid and cost-effective biosensor platform for the detection of ARDS is needed. We developed a paper-based multiplex sensing platform to detect human neutrophililastase, NE, proteinase 3, PR3, and matrix metalloproteinase 2, MMP2. By monitoring the levels of these proteins in infected mice after intranasal administration of lipopolysaccharide, LPS, we found that they play an important role in ARDS. The paper-based sensor uses a colorimetric detection method based on the cleavage of peptides linked to magnetic nanoparticles. This allowed us to distinguish between the presence or absence of the target proteases. Multiplexing of NE, PR3, and MMP2 was tested and compared after 30 minutes, two hours, four hours, and 24 hours of LPS administration. The multiplexing platform of the three analytes resulted. This article was authored by Nuhakalat al-Akmimi, Dana C. Alame, Kasim Ramadan, and others. We are article.tv, links in the description below.