 This research paper outlines a new, efficient, and cost-effective high-performance liquid chromatography, HPLC, method for analyzing fire pentylsodium, though, in intravenous anaesthetic drug. The method uses a mobile phase consisting of potassium dehydrogen phosphate, KH2PO4, solution mixed with methanol, 40 to 60, at a ratio of 4 to 1, with a flow rate of 1.2 milliliters per minute and a detection wavelength of 230 nanometers. The method exhibits excellent linearity, repeatability, sensitivity, selectivity, and accuracy, as well as a short analysis time. It also meets the requirements of international conference on harmonization, ICH, guidelines for validation of analytical methods. Furthermore, this method can be used to analyze though levels in pharmaceutical preparations, ensuring their safety and efficacy. This article was authored by Mustafa F. Olhakani, Norhan Ahmed, and Mohamed H. A. Hassan. We are article.tv, links in the description below.