 Hi, we are from Biotrans A-Star Singapore. Our research interests are engineering microorganisms to produce flavor and fragrance compounds. Here we present you a short video abstract of our recent work on plug-and-play modular system to produce apocardinoids. Some examples of apocardinoids are alpha-beta-ionone, which they have very sweet and pleasant smell, and the retinoids, which is the vitamin A. The challenge to achieve high titers of apocardinoids in a heterologous host is that, firstly, the synthetic pathway from glucose to apocardinoids is very long, and secondly, some of the enzymes are membrane-bound and extremely inefficient. In our work, we overcome the challenge by using modular metabolic engineering approaches. Since all the apocardinoids were derived from the same precursor, lycopene, we first obtained the parental strain, which is able to accumulate high concentrations of lycopene. Based on the parental strain, we further overexpressed the lycopene cyclase and carotenoids cleavage dioxinase to produce alpha-beta-ionone or retinoids. The modular approach also allowed us to identify that these two enzymes are the main bottlenecks. In order to improve their activity, we further used the enzyme engineering methods such as n-terminal modifications and enzyme screening, and this method effectively improved the apocardinoids tighter by more than 54. We further tested the strain in biorectrase. For both alpha-ionobeta-ionones, we have produced about 3.5 gram plethora, which is about 100 times more hair than perversely reported. More importantly, our system is a plug-and-play modular system, so we can literally adapt our system to produce other apocardinoids, such as saffron proteins. In sum, we have successfully underneared a series of strains that overproduce alpha-ionobeta-ionones and retinoids, and our study laid foundation to sustainability produce other important flavor compounds. Finally, our study proved that it is very powerful to combine modular undernearing approach and enzyme undernearing approach, and this strategy might have a broader application.