 The study presents Slingshot, a novel method for inferring cell lineages and pseudo times from single cell gene expression data. The method accurately identifies biological signal for one to three branching trajectories and infers more accurate pseudo times than other leading methods in a simulation study. Slingshot is a robust and flexible tool that combines stable techniques necessary for noisy single cell data with the ability to identify multiple trajectories, which is critical for identifying dynamic temporal gene expression. This article was authored by Kelly Street, Davida Rishow, Russell B. Fletcher and others.