 Hello everyone, my name is Xiang Menglei. I'm from Tianjin University. If you are puzzling about the low deter of one macrophonator product and 34 new tools to employ it, please pay attention to us, because today we are going to provide a new and effective method. FK506 is the most important drug used to prevent rejections of transplanted organs and in the treatment cell autoimmune diseases. It is also one of the world's best-selling medicines. However, the low deter has become a bottleneck for its application and industrialization. In order to enhance FK506 yield, considerable time and effort has been expanded, including medium optimization, classical strain improvement, and gene manipulations. All these measures are laborious and tedious and sometimes require long and unpredictable durations, to date little quantitative improvement has been achieved because of the limited understanding of the intracellular metabolic activities. To address this issue, we provided a novel method. First, we choose two medias in which the strain-disciplined distinct production capabilities and fermentation characteristics. Cell samples with different specific productivities were harvest from the two medias and profiled. On the base of metabolic profiling analysis, 13 key meta bodies involved in five pathways were determined to be directly correlated with FK506, and they served as the potential regulation points for FK506 over production. To verify our analysis, these meta bodies were added into the medium, and the teacher of FK506 was improved significantly. Finally, a rational feeding strategy was proposed, and the targeted product was increased by 61.4 persons. What's the most important of that? A deep understanding of our production mechanism of FK506 has been achieved. If you are interested in our research, please log by engineering and by technology for more details, or you can contact us directly. Thank you.