 Hello everyone, my name is Ignacio and I will be presenting some work that we've been doing with the election team to support the submission of viral data to ENA. So this process started about a year ago to try to tackle the common challenge, which is the access to data. So I was observed here in Belgium that normally their labs, especially the clinical labs, we're doing submissions mainly to GSA, and especially submitting consensus sequences of viral data. And pretty much no one was doing submissions to ENA, even though, yeah, we can point out quite a few extra benefits of it. First of all, it's a fully open platform so pretty much anyone can submit and retrieve data for it. It has a quite well structured metadata that described everything that they submit. It can take, for example, low rates and not just consensus and sequences and genes, and it's already, for example, integrated into COVID-19 data portal. So any kind of analysis that makes use of this portal to fetch public data, it will benefit from any submission that is being done to ENA. So the starting goal that we had was to get more high quality data into ENA. And to do this, we decided to create a galaxy submission tool, which will focus mainly on the submission of rates. The idea, in general, was to actually lower the technical barrier that was present for bike submission, especially. This is mainly because it was quite complex to specify the metadata, especially by using the ENA web interface. And the idea of creating a galaxy tool was to benefit from the galaxy graphical user interface, which is really easy to use. So the idea was to use a tool or create a tool that will involve a single step submission that is pretty much just one click. So it would should somehow manage the ENA credentials and the management in the galaxy context. So we will be using this CLI tool that is linked here. And the idea also about the metadata management is that the users would input pretty much all the metadata by using by filling it in plate spreadsheet that we created with some control book allies. And then by using the galaxy data types, for example, the collections, it will be really easy to do the bug submissions. So this is basically an overview of the tool that we we designed. So basically the inputs are the raw rates and what I mentioned before about the metadata spreadsheet template that should be filled by the users. So I think that is within the degree box, the gray box is it's transparent for the user. So it does any kind of people seem that is required. It just runs the ENA upload tool. And it also it's important that it fetches all the accession numbers that are retrieved from ENA and it reports these to the user so they know how to access the data once it's just on ENA. So, to provide access to these tools, we have three different options. One is the users can access to COVID-19, which is an instant that is specific for version institutions. So they need to request access. And once they are there, they have access of course to all the computational resources. When they upload the data, they have access to some prepositing tool, for example, to clean the human rates, and they can do the submission using this tool. And this submission will be used, will be done using the exhibition broker in account. So all the submissions done through this instance are linked with the same credentials. So the option that is available is we created a small ENA upload container, which contains not just this tool, but also all the preprocessing tools that are or might be used, might be needed before the submission. So in this case, you just don't know the container and it will run the galaxy instance locally. And you can just access the tool and you can fill the ENA credentials in the user preferences. In this case, you only need just one dependency, which is talking, of course, and you can run it locally. So the other options, of course, using the public instances, this is a public tool. So it's already available in several instances, for example, in EU and users will be in this case anyone can register and upload the data. And in this case, also, you need to fill your ENA credentials that you get from ENA website and your user preferences. And in this case, yeah, of course, the submission is done using this specific credentials. So we also did quite a lot of work in terms of documentation. So I wanted to highlight some about this. You can visit particularly this first site to see a really detailed guide of how to do this mission, how to use this tool, and the different options that I just showed. And also here you have a lot of documentation related with the, for example, the preprocessing tool, and also the container itself. And that's about it. Things, of course, will be funded in agencies and to all the people that did their work.