 The Network for EcoHealth and One Health, NEOH, have proposed updated core competencies for the One Health workforce. These competencies are grouped into three main areas of skills, values and attitudes, and knowledge and awareness. The competencies are intended to be applicable to stakeholders from various sectors and across all levels, and can be used to evaluate and enhance current curricula, create new ones, and inform professional training programs at all levels, including students, university teaching staff, or government officials as well as continual professional development for frontline health practitioners and policy makers. The competencies are also aligned with the new definition of One Health developed by the One Health High Level Expert Panel, ILAP. This article was authored by Gabrielle Lang, Eleanor Duffy, Neil Anderson, and others.