 The National Center for Advancing Translational Sciences, NCATS, has identified the need for increased research with priority populations to reduce healthcare disparities. To address this issue, the University of Illinois Center for Clinical and Translational Science, UICCTSA, developed five priority populations toolkits to help researchers better understand and engage with these populations. These toolkits include curated materials from scientific literature reviews and internet searches, as well as feedback from user surveys and analytics. Since their launch in 2016, over 387 unique users have accessed the toolkits, most often to improve research recruitment efforts. This shows promise for increasing knowledge and readiness to work with priority populations. Additional toolkit development and evaluation of effectiveness are recommended. This article was offered by Kevin Ragh, Alicia K. Matthews, Gabriella Pina, and others.