Exploring One-shot Kinetics™ and Small Molecule Analysis





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Published on Jan 28, 2013

For more info, visit http://www.bio-rad.com/yt/12/proteonw....
David Myszka, a pioneer in biosensor research, relates how Bio-Rad's ProteOn™ XPR36 protein interaction array system is used to characterize biomolecular interactions. With a single injection into the ProteOn system's crisscross flow system, response data was collected for six concentrations of each analyte over six target protein surfaces. This One-shot Kinetics approach to kinetic analysis improves throughput while generating high-quality data.

Prof. Myszka introduces the features of the ProteOn XPR36 system and describes how data are obtained, analyzed, and interpreted, using the work with a set of small molecule--enzyme interactions in his laboratory as an example. The results illustrate the potential of this parallel-processing biosensor to increase the speed of kinetic analysis in drug discovery and to expand the applications of real-time protein interaction arrays.

Presenter: Prof. David Myszka
Center for Biomolecular Interaction Analysis
University of Utah


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