 We describe a tool called REF genes that allows comparing RTQPCR measurements with specific referent genes. RTQPCR measurements often show very high variability in their expression levels compared to the transcriptome. To normalize RTQPCR measurements, it is important to choose appropriate referent genes. REF genes can be used to normalize RTQPCR measurements by searching for specific referent genes. This article was offered by Blula Stefan, Kalei Jiva Luba, Babru Feeb, and others.