 Salivary analysis is a rapidly growing field with great potential for advancing healthcare. It is a non-invasive technique that utilizes biomarkers found in saliva to provide valuable information about an individual's health status or disease progression. This review summarizes recent developments in salivary analysis, including its applications in diagnosis and monitoring, and discusses the challenges that still need to be addressed before it can become a mainstream tool in healthcare. This article was authored by Tsai Zhiliou, Xiaofeng Chen, and Ning Fu.