 ANVIO is a comprehensive analysis and visualization platform for microbial genomes in metagenomic assemblies, with interactive interfaces that link BOMIX data from multiple sources into a single, intuitive display. It offers automated and human-guided characterization of microbial genomes, and its extensible visualization approach distills multiple dimensions of information about each contig. ANVIO is an open source platform that empowers researchers without extensive bioinformatics skills to perform and communicate in-depth analyses on large OMIX datasets. This article was authored by Amorad Aran, Oskan C Ezen, Christopher Quintz, and others.