 Biofuels are environmentally friendly renewable fuels that can be directly used in diesel engines. However, some drawbacks such as a higher density, viscosity, a lower calorific value, and an increase in nitrogen oxide, NOx emissions, have led researchers to explore ways to improve the physical properties of biofuels and reduce harmful emissions. One approach is to blend oxygenated additives, such as alcohols and ethers, with different generations of biofuel. Research has primarily focused on alcohol additives with first generation biofuel, but few studies have examined the effects of oxygenated additives with second generation biofuel. Second generation biofuel is derived from non-edible sources, such as neem, jetropha, and califilm inofilm seeds. These plants produce high levels of oil, making them attractive candidates for biofuel production. Neem, jetropha, and califilm inofilm biodiesel blended with methanol or diethyl ether were found to reduce carbon monoxide, CO, and hydrocar. This article was authored by Saad Ahmad, Ali Turab Jafri, Mutibul Haq, and others. We are article.tv, links in the description below.