 Organs are three-dimensional, 3D, are miniaturized versions of organs or tissues that are derived from cells with stem potential and can self-organize and differentiate into 3D cell masses, recapitulating the morphology and functions of their in vivo counterparts. These organs offer advantages over traditional two-dimensional, 2D, are cultures, including preservation of parental gene expression and mutations, as well as long-term maintenance of the function and biological characteristics of the parental cells in vitro. Additionally, organs can be used for drug discovery, large-scale drug screening, and precision medicine. Furthermore, organs can be used to model hereditary diseases that are difficult to model in vitro through the combination of genome editing technologies. This review provides an introduction to the development and current advancements of organoid technology, focusing on its applications in basic biology and clinical research. It also highlights the limitations and future perspectives of organs. This article was authored by Sikyung, Haijihu, Hengchengkang, and others. We are article.tv. Links in the description below.