 This study investigated the prevalence and genetic basis of antibiotic resistance to macrolides, lincosamides, and streptogramins, MLS, in enterocacus SPP, as well as the correlation between MLS resistance and biocide resistance. The study found that 76.8% of the enterocacus SPP isolates were resistant to MLS antibiotics, and the most common resistance mechanism was constitutive resistance to all MLS, CMLS. Additionally, the study found that the efflux encoding genes MEFE and MEFE were significantly associated with increased mics to both MLS antibiotics and biocides. Furthermore, the study demonstrated that the efflux of resistant isolates was significantly increased when they had higher mics to both MLS antibiotics and biocides. This article was authored by AMR Salim Abu Laila, Tariknafia Alherbi, Jauhar Al-Nazi, and others.