 This study examined the use of a novel biocompatible electrospond nanofiber scaffold made from poly, caprolactone, PCL, and chitosan, CS, combined with nigella sativa, NS, seed extract. The scaffolds were evaluated for their physical properties such as poor size, surface morphology, and antimicrobial activity. The results indicated that the scaffolds had a homogenous and bead-free morphology, with an average diameter of 81.19 nanometers. Furthermore, the scaffolds exhibited excellent antibacterial activity against both Staphylococcus aureus and Escherichia coli, while maintaining cell viability. These findings suggest that the PCL slash CS slash NS nanofiber scaffolds could potentially be used to treat and prevent microbial wound infections. This article was authored by Kasim Shaker Khadim, Najmeddin Abdelmullah, Hassan Al-Kharagli, and others.