 Honored for me and thank you for this opportunity to be to speak to you in this links webinar series. What you see here is the campus of CNPM National Center for Research in Energy and Materials. This is a non-profit organization is located in Campinas and we have a contract with the Brazilian Ministry of Science and Technology to administrate these four laboratories. So the first laboratory you see here is LNLS, they gave birth to this center and we started with the second generation lab source, UVX we used to call it, is deactivated now and the major single source now is Sirius that I'm going to talk about but before that I'm mentioning also the other four laboratories, LNBio is leading facility in structural and molecular biology, drug development into cancer and cardiovascular disease and pathogenic biology as well. LNNANO is the most comprehensive lab in nanoscience and nanotechnology here in Brazil and it has a complete set of electron microscopes even a cryo electron microscope is the only in the country that had been doing a research on viruses as well and LNBR which does research in development in microbial platforms for the production of biorenewables and in industry scale to be honest and they use structural biology and material structures all the way to investigate the transformation, sustainable transformation of biomass into different chemicals. So my talk is going to be divided in basically five parts. I'm first going to give you a hint on the evolution of our project from 2015 to 2021. I'm speaking something about the 4G sources and some of the scientific program and how do we provide user support and some of the R&D developments that we did with industries. So what I'm going to show is mostly results from other synchrotronals and commissioning results from CDs as well. So please feel free to ask me questions about these. These are very new commissioning results. But let me start a story in more or less about 2013. So the project itself started in much earlier, but in about 2012 the MAC meeting, the machine advisor committee, advised us to aim for a sub-nanometer radian synchrotron and used in the multi-benachromat technology that was being pioneered by Max Ford, Nund. So that was a big challenge. But we decided to take this challenge and follow in your footsteps and face the many engineering challenges that were coming ahead. So this is an overview of the, I don't know if you can see my mouse, of the, in 2013, for the different projects that we had, ESRF now has the EBS synchrotron, Max Ford of course was the first MBA synchrotron in the world, and the first more fourth generation so-called. And now I'm going to talk about CDs, this project here in the south of Brazil. And we know there are many other projects coming ahead, and this is a very exciting moment for the synchrotron community. So, after these discussions and the project change, we started that basically in 2015 that's when we had the first on and started the construction in the, yeah, it's a greenfield site as well. So you can see the evolution of the structure construction. And in October 2018 was more or less when we had the building ready for installation of the machine. That's when we started the, the storage ring, the booster and Linux installation. So what you see now, it's a small video, yeah, that shows the internal parts, our Linux, that was not built here, but everything else except for the Linux was built in this campus with our own technology and with many partnerships with Brazilian industries. That was a key aspect of the project that we developed the know how, and we transferred to the local industries, so that they could build parts for this new synchrotron. So, yeah, we are not traveling at the speed of light, but you can have a fast overview of the internal parts of this storage ring. Use the neck coating technology for vacuum parts as well as most of these new generation synchrotrons. And what you see now it's, we have several extended bean lines that go up to 150 meters. This is the front end so there's a part that connects the storage ring to the external part. That's where we are going to now. But before, let me give you a timeline since this installation and the first commissioning results. So back in 2019, that's when we had December 2019, that's when we assembled the first beam line that was a micro tomography. First assembly of the micro tomography beam line and with 30 micro amps of current so orders of magnitude less than the design project, we were able already to do the first tomography back back in the end of 2019. And since then, then we had accumulation with non-linear kicker. But as we were starting to have the commissioning, the world was halted by the COVID pandemic. And we had to move some steps down and reduce the amount of stuff in the campus just for very crucial things that had to be maintained. And as in this low space, we tried to ramp up again some of the activity still with about 25, 30% of the staff only on campus. Most people working remotely. And we had a challenge to actually assemble and commissioning a beam line basically doing with a majority of people from, you know, this online platforms. We didn't have pickups like this one we had today, but that was very challenging in fact because most of the, you know, challenges discussions and the anxieties and the, and the also the celebrations they were done basically through the computer interfaces was actually quite different from what we are used to do in a commission. So by July 20, we had the first commissioning technical commission of the coherent defraction image in being lying. And since then we've been ramping up to the current and starting new with technical commissioning of beam lines. The beginning of this year now we had a major realignment, and now we expect to start working with 100 milliamps and start using commissioning. But before I talk about this, let me just give two slides about what we define as fourth generation storage ring light sources. Basically, we, we understand that a single election is in an in an undulator and a standard source produces radiation that we can describe having a size that depends on the size of the undulator and the wavelength of radiation and a divergence that also depends on the quantity of basic one electron emission radiation, and as soon as we have an electron beam, then the really that has some size and divergence, the final being delivered to the beam line is a convolution of this size and divergence. And the major challenge here given certain wavelength is that we can match the emitters to the desired wavelength. And then we can extend their x-rays for instance in the range of 100 nanometer radians, and also to match the beta functions is a characteristic of this later into the way at the length of this undulator. So, when we match these two conditions we reach what is called the fraction limited for this wavelength, and then we can extract part of the coherent radiation from the source. Very simplistically, we can think about the generations of storage rings, right, we had the second generation here. The size of the transverse size of the beam, the electron beam is what has been, we talk about a lot about brilliance and about the emittance, but effectively for the science that we're doing, what has been reduced since the, you know, we improve generations of storage rings is this size so we can think of second generation of the order of one millimeter size that was the old secret from we had a third generations as 100 micrometer and as we go to the multi band technologies here headed by Max for we reach beams of the order of 10 microns so we can think of one order of magnitude reduction of this transverse size as we move to the down in technologies. Now, why is this important, because first, if we want to extract coherent radiation, the amount of coherent radiation fraction that you get from this beam line is inversely proportional to the size so there go. The most important point is that if we want to use a small lambda, we rather have a very small beam in the horizontal size, the direction, the vertical direction is typically very small, but this allows you to do coherent the fraction X-ray photon correlation spectroscopy and several other techniques that need coherent beams. Now, also because the beam size is small, you can demagnify it much easier because basically it's proportionality between the small beam size and the source size, and this opens possibilities using the nano probes, extreme condition environments where you have tiny environments and you have to penetrate them with a small beam and a very low divergent beam if you want to do the fraction for instance. So these conditions is the kind of small beam sizes what allows us essentially to do all this new science of four generation beams. Sirius was a machine designed to cover this techniques, it was implemented for high coherent fraction, but we have a slight different design from Max 4 for instance, and we have this separate bending magnets in different benches, and one characteristic that allows us to do is to extract also infrared radiation, UV radiation from this magnet. So this is an example that in the fourth generation syndrome, we're still using conventional techniques. Why this is important? Because we believe science is done with this complete set of techniques all together, so it's an enterprise where we are basically looking into multiple wavelengths, multiple scales, and their interesting system in natures are systems that you have to look into multi-modal multi-modal space time scales. So it's a machine with a 518 meters diameter, 3GV, pretty similar to Max 4. The lattice was a five band achromat, we expect to reach 350 milliamp, as I said, we are still in the 70 milliamp. And otherwise, the emittance is about to reach 150 picometer radian as we install the undulators. Now, this is the set of beam lines that we are planning for these first two phases of installation of beam lines. And this is where I'm going to focus my talk now. So essentially, describing what are the scientific opportunities that we hope to find in this kind of new light source. And I'll give you some flavors of what we've been doing. But if you need to find more details about the description of these beam lines, you can find in our website. I basically divide the beam lines into themes. Of course, these are beam lines with broad spectrum of not only radiation but of applications. But they essentially cover different spatial information from the resolution to the field of view. And they also provide the different information from structural information all the way to electronic information. But for instance, this beam lines that we think dedicated to quantum materials, we are installing beam lines for unresolved for condition resonant diagnostic x-ray scattering, photo electron microscopy, and extreme conditions for the biological or living systems. Then we have the traditional protein crystallography and socks and circular dichroism, but also CDI, XPCS, and nanoFTIR, as I mentioned. Whereas for the functional materials or the genius hierarchical matter that basically joins these two scientific scopes, then we have a broad range of x-ray beam lines from x-offs to the fraction to nano probe and from the interpolation function from the fraction. Now, but let me start with a bit of a philosophical question, let's say. So, typically, when we discuss what syncretons and what accelerators do, right? It's a tendency to think that, okay, reality is made of these inventory particles, and then the quarks, and the laptops, and the gauge bosons that made them interact. Now, being virtually in our days and discussing with everyone through virtual worlds, we get philosophical and we've been asking, what is real? Is this fundamental particles real? Or are chairs and people and ideas and love, this is real and these particles are just the useful fiction that we tell ourselves. Now, I like to think that this is, of course, this is a problem for the philosophers, but nevertheless, we still have lines to draw where one would talk about viruses and cells and mutations and organs. How do we draw this line of what is fundamental and what is reality and what is just a manifest image? Well, I like to take the philosophical view of the philosopher Daniel Dennett, that things that describe patterns, they are real, they are the touchstone of reality. So, if you can predict something with a model, then it's real. And because we are pattern finders, we're designed by evolution to find these patterns, we just need to think of higher level structures that built these descriptions. So, essentially, what I'm trying to argue is that even though in single terms and we don't necessarily look for these elementary particles, we also have to find the standard models that allows us description of nature. So, let me start with quasi particles. So, because this is the closest we have to the phenomenon that we observe in elementary fields. So, but to different proclamizations, when we look at electrons in correlated material, typically describing them as single particles to a very entangled state of of electrons is pretty hard. And in fact, doesn't make much sense because as the Fermi liquid theory from described by Landau, most of these excitations that we see and condense matter. They behave like particles, and they manifest themselves so the manifestation is like particles, but with different kind of characteristics from the electrons. So, we can they can either be, you know, phonon particles, and they their guests can describe thermodynamic properties of matter or Magnus, and the gas of Magnus describes the thermodynamic properties of a magnet as well as a gas of particle describes gas of molecules describe the thermodynamic of gases. And, and then we have different all many different kinds of particles that build this new reality spin ons, holos, scrimmions, and all these quasi particles they are part of this reality between mille-electron volts and kiloelectron volts. Let's take for instance this very nice result from the literature from resonant elastic x-ray scattering, where we observe from experiments the reality of particles that carry spin, but no charge, or particles that charge excitations that carry charge but no steam, or the ones that carry orbital moments but neither of them. Or even when we look for single particle properties from on-go result automation, that we observe particles that can be predicted from, you know, to maybe they exist in the elementary fields, like Majorana fermions, that may be necessary to explain the mass of neutrinos. But in condensed matter, they have just become real because these are the patterns we observe. And the surface of some topological superconductors, for instance, they show aspects and patterns that just behave like this elementary particles. And so the idea here is that you need these patterns and you can probe these patterns through synchrotrons to describe your reality. So, and we don't need to wait for nature to create these fields, we can actually search for materials using synchrotron techniques like extreme conditioned techniques like this one to observe phase diagrams where we look for overall behavior on material until we find a phase that describes a topologically non-trivial material where we can see these excitations as a reality in these materials. So this is the first case of quantum materials. And this is just a very fresh result from the Emma beam line. So it's a beam line dedicated to high pressure diffraction and high pressure and high temperatures and very low temperatures and magnetic fields of the order of 11, then 10 Tesla and pressures of the order of Terra Pascal. We're commissioning these beam lines, but you can see already the results from the beam line that we can draw phase diagrams and phase changes from the high pressure diffraction experiments and follow up different aspects of this materials as we probe the phase diagram. So basically now in the phase diagram, we're not talking about quasi particles, we are describing the nature to order parameter or something in a higher level. Now, I now summarize this area. Basically, we have the suit of beam lines for quantum materials numbers off of the mission like the case that I described ricks like the other case I described for this collective of excitations XMCD and PIM and extreme conditions. Now, when we move to, let's say, the animate matter, and I described phase transitions. And, and I argue that we look from a different perspective than we would look from elementary particles, or even from quasi particles. When we want to describe the living matter. It's, it's not helpful to start from elections, even from quasi particles, or even from phase diagram simply, we need to start from agents from proteins that have actually intentions they have function they have structure, and we have to understand from the structure. So let's put together, and how the dynamics brings life from the separate processes and from adaptation of this separate processes that matter can, you know, find different configurations where it can absorb or can reflect completely and can be stable in different environments. In the building blocks of these constructions, they have to be in a higher level scale. What you see here, for instance, is a very nice combination of structural biology, microscopy and biophysics by these very nice images from Dave Goodsell, all integrated in what we believe to be a complex fluid of a cell. Now, we're far from understanding how life emerges, but if we want to have a chance to understand this processes, and to be able to predict future of and make this transition to understand life from from molecules from this building blocks. We need more than just structural biology. We need dynamics as well. So, and this dynamics can be called in different times scales. Now protein crystallography is a well standard technique already in many synchrotrons. And in fact, this was our first being line to start operating. And this been line was commissioned and is already accepting users, especially for his studies with COVID. That's where we started and now we are accepting users in other areas as well. But the next stage of this been line will be serial protein crystallography with my cabin line. And there's a very nice trade off of obviously free electron lasers in this time scales off into seconds and picoseconds they are unbeatable. But there is a millisecond to second time scale, where we can extract lots of understanding about the dynamics of conformational changes or proton uptake that build up this higher level dynamic structure of living matter. And so this is the first result from the Monica been line. It was in fact, the three protein the three CL protein from the COVID. This is first commissioning very soon after that we started having users so as you see here this is the first picture of users coming to the been line already testing target drugs into COVID proteins and also testing different ways of doing sad and obtaining electronic maps of different proteins into the low energy side of the spectrum. So this is brand new commissioning results. And as I said, this been line is already available for especially for shipping samples and using the in the syncretron. Now, as I said, just building blocks of proteins are not enough to describe to you what life is. You need to build and understand you negotiate with this higher level structures and understand their interactions, their dynamic. So for that, you need boundary conditions like the cell structure and where the elements are located. And so, in this sense, correlative nano probes and x-ray fluorescence imaging combined with coherent diffraction imaging. They provide this higher level and organization structure where you can input into those simulations in the future we'll be able to join structures and put it all together to this boundary conditions. This are results done of a 3D cell structure done at APS from our colleagues. And it's a very interesting result. This took, of course, several hours to be done. And until it becomes a user widespread technique for understanding two dimensional structural cells, we will need the higher coherent flux from fourth generation syncretrons. But as soon as we understand this, right, then the next step is, okay, what is the dynamics that is built in into these interactions. And this also comes from coherent probes and the coherent fraction of new syncretrons. For instance, let's have a look at how new results from the dynamics of how proteins interact. So in a larger time scale, a space and time scale of organization, we understand from several time resolved life fluorescence experiment that face separation, for instance, where these proteins separate explain many aspects of cell organization in the cells and their function. And now several research groups have reported that this mechanism condensing hundreds of proteins. They carry functions like transcription, like immune responses, and even reproduction of the SARS-CoV-1, SARS-CoV-1 viruses. At the same time, face separation may cause disease when it goes wrong and form toxic those other bodies. So understanding this dynamics is also part of the new science that will come from this fourth generation syncretrons. And now this is a very recent result that was published a few months ago that uses X-ray photon correlation spectroscopy from the syncretron, it's a third generation source, but it can show already signatures of separation between kinetics and dynamics of protein condensates. They can evidence even two very distinct time scales. And some of the phenomenon that we understand that may be necessary to, you know, give the next step for adaptive dissipative matter. Basically understanding memory, understanding adaptation, understanding all the aspects that we believe are built in into live behavior. Now these are results from XPCS and it's something that will be part of our scientific program as well as, you know, other syncretrons. So to give you a flavor of what's going on, this is our coherent diffraction imaging beam line, the beam line that Aline works and she was just here helping us. Thanks to her, we were able to start this discussion. And this is one of the first results where they take the zeolite particle and that was obtained by coherent diffraction image is a nanoparticle. And so this is the detector inside the tunnel of the beam line, one of the R&D developments done here in Brazil. It's a larger 10 megapixel detector that goes into the vacuum tunnel. And once, you know, we have the sample isolated, the coherent diffraction patterns, they show very nice speckles all the way through the acquisition patterns. And we can see actually the visibility of these patterns is excellent, it shows already the characteristics of high coherent fraction from the syncretrons. Now, we need a very fast detector and a high dynamic range detector as well. So this is one of the key developments is flagship development that we did based on the metapix ships from CERN. We built this 144 ship detector R&D detector that goes into the Caterete beam line. This is a flavor of what's going on in our commissioning. Now, putting it all together, these beam lines from living matter understanding, they also make a suit of beam lines that can tell us about nano FTIR with it's basically as non-technique, this Caterete beam line for CDI and XPCS and the protein crystallography and socks beam line. Now, when we put together aspects of an organic matter and living matter, then we come into the materials that behave completely differently because it's something created by evolution. It's a functional and like the soil for instance is the best example is one of probably my favorite examples of how science is going to, you know, tackle our understanding of our current environment and help us, you know, go to our future without destroying the planet and destroying ourselves. Quoting Frederic Albert Fallou, in nature there is no subject more worth to be considered than soil. It is this domicile for humans and by itself it originates, nourishes a multitude of beings, which the entire creation and our existence ultimately lasts. So now, when we go into this soil structure, for instance, then building blocks like just prudence are not enough, we have to build our standard model for an even higher level structure. And our, you know, elementary blocks should be hydroxides in organic samples, minerals, cementing agents, phylosilicates, bacteria and fungi, root hairs, organic dead matter, and different concentrations of ions distributed in the pores of the soil. Now, this whole complex high ecosystem is intrinsically the region use in hierarchical and the properties come from this web of interconnections between living matter and physical chemistry of fluids in pores granular matter. Now, to make progress, and we also need to understand, for instance, the interface in between soil and the plants, the living organisms that live in the soil. Now, life on earth is sustained by this small volume surrounding the soil of roots. This biome is called rhizosphere. And we have different land scales and time scales that that make this biome living and allow flow and transport and reactions, and everything that brings life on earth. Now, probably is the most relevant biophysical and biochemical processes that happen in soil, they happen here on this rhizosphere. And we've been also commissioning results from the different systems. This is an example of data from our beam line, the microtomography beam line. What you see here is already part of what we intend to do in the future. This is a tomography from a plant inside the soil, where we can study how porosity structure of the roots and the hairs, how they all come together to distribute nutrients in between the systems. Now, we are planning completely, I'm sorry that this picture was above, but we are designing systems that we can use plants in situ and we can study not only plants, but the soil as well using fluorescence tomography, the fraction. So this is special devices that were designed to allow us to study the rhizosphere on soil. It's called the rhizomicrocosm. Some examples of what we've been doing also in terms of 4D tomography, what you see here is time resolved tomography where we see the flow of liquids to a porous system. And all these renderizations, it's something also that we've been taking a lot of care and is providing users with tools that we can deal with this mongoza density of data. Not only that, but providing ways of interacting correctly with the data. This involves not only data taking, data processing, but also the way we cognitively understand our data. So this is brand new, these are pre-computed ambient occlusions and techniques that allow you to understand the three dimensional data and interact better and provide new clues for new hypothesis and new experiments. This is another example of this fluid distribution that was done into the Mogno beam line. And the Carnauuba beam line, which goes to the other end, is the Nanoprobe beam line. I'll give you now just a quick tour inside our synchrotron, where you can see this is the longest beam line we have. And then our flagship beam line for Nanoprobe. You will have two experimental stations, one dedicated to submicron and in situ environments like the Rhizocosm, and one dedicated to nanometer resolved imaging. So what you see here is a complex structure of detectors all surrounding the sample stage fluorescence, diffraction, and coherent diffraction imaging, and we will also have different probes like x-ray optical luminescence. Okay. These are recent results. We just started to commission this beam line. What you see here is the sample stage. This is one of these detectors that we developed based on many peaks. And the results that just came out from the beam line, these are commissioning results. First, the steps that we did, picography and fluorescence from a soil sample, where we can see with sub-micrometer resolution, I'm sorry, the distribution of different elements. So the idea is to, of course, interleave these two results and use picography and fluorescence tomography to obtain a three-dimensional mapping of elements in soil or other systems with nanometer resolution. So with that, then I conclude the suit of beam lines for these functional heterogeneous and hierarchical matter from the chromography beam line that I showed to nano probe and diffraction PDF and exhaust beam lines that we plan. So altogether, these are the suit of beam lines to cover different aspects of matter and different spatial resolutions and field of use. As I said, the description of these beam lines and the ones that are in current installation commissioning are the ones here with black line underneath. But you can get more details on our website of how these beam lines work, their specs. Now, we've been commissioning, this is current stage of our commissioning. We are doing basically are getting close to the focal length and the focal sizes that we expect for these beam lines. Protein crystallography is a beam line that we can adjust the focus from micrometer to 100 micrometer. The plane wave beam line is getting close to also to optimal. Some of these beam lines we still limited by the measurement device, but we are close to installing this first six and commissioning and as we commissioned the accelerators as well. So this is something brand new that we've been doing both of these stages together. This is an example of R&D that we did with Dutch company Mechatronics. This is the monochromator that we've been using in many of our beam lines. It's a high stability non monochromator it's necessary for maintaining the beam side effective beam size that that we work so hard with accelerator to reduce the levels of nano focusing. So this is an example of accelerator that we will have a nano radiant stability even for fast scanning. And I give you just one example of what we did recently. It was an exhaust spectrum for standard samples synchronizing the under later with the monochromators, and we can achieve this nano stability with high energy speeds, and even do examples that are very textbook with fast scanning of fast energy scanning. Now, to conclude, we are not going to into details but we expect that series is not going to provide only the acquisition sample preparation like most synchrotrons, but we hope to extend also sample synthesis and pre characterization tools for the users, and also modeling computing tools so we expect to open up the spectrum of what the users can do in synchrotrons. This is just an example of labs that we have installed available for users. This is a thin thin thin film growth lab. We have other labs like a feed device to cutting samples for now probe and high pressure and many other labs that are going to be available as well as the computer infrastructure so we have clusters of GPU and CPU that can I'm sorry this is not working but basically is a data center where the users can access the computing power during their experiments and after that experiments as well. So all the infrastructure the software will be accessed remotely from the users after the experiments. And we also like to point out that most of our development and purchases were done here with Brazilian companies and international companies but most of the purchases done in the project they were done in the country in Brazil that was a prerequisite for the project as well. So, finally, my closing remarks is that we had to adapt installation and commissioning during the dynamics of course, but it's working quite well to the very integrated and collaborative team this is essential for the success of any facility. The online and machine commissioning are happening simultaneously. This is challenging but it offers also great opportunities for understanding the different aspects of single-tron science. We're close to nominal emitters of 250 computer radian. We still need to implement fast orbit feedback, and we expect to by the end of this year to be working with pop up at 100 milliamps. We're still not the nominal current, but we will allow most of the experiments that we need. We have had lots of involved developments with external companies. We still need many of them to be further commissioned to require like to be commercial products, but they will soon be available for other communities as well. We also implemented support infrastructure for different labs for chemistry, thin films, extreme conditions, the feed and the cryo preparation labs are just being installed and high performance computing is already operational and being used by users. We expect the first coherent diffraction imaging results are very promising, and we expect to bring user commissioning with shipped samples. We are still not allowed to bring people to the campus, but we expect in the next couple of months, so stay tuned. We hope to open for beam lines for regular submission of proposals. We're not regular yet, but user commissioning. So basically, trying samples that are challenging, and also the support labs for sample preparation and the high performance computer so computing system so we'll open for users for user commissioning in the next couple of months. So with that, I thank you all. And I wish to some time to hear in Brazil, and to have you also take advantage of the kind of tool that will provide advances for science. Thank you very much. It was such a great presentation. And yeah, so we're a little bit late for today's webinar series, but I think it was really worth it to listen to your presentation until the end because it was really, really interesting. Thank you very much for having me again. So we have questions from our audience, and I will, yeah, quickly go through them. And please, if you have more questions, just write them on the common chat. But first question. So, since the machine is based on the max for concept. How much collaboration exchange is there between the two facilities. Thank you for the question. The is, I think that yes, most of the new synchrotrons are all based in this multiband acrobat technology, which was spearheaded by Max for, and there was many different aspects of this collaboration in fact, which was one of the, the pioneers of this technology that was part of the machine advisory committee that proposed that CDs should aim for a higher standards into this fourth generation syncretron now we do have collaborations with in this community is not a large community of synchrotrons and we all exchange ideas and designs. And I think that more should come of course, but yes, it's a different it's likely different aspect because it's just the Max four is a seven band acrobat series is a five band acrobat based on high magnetic field a pose also super bands of 2.2 Tesla so technically, they are slightly different, but of course we will, we always learn a lot from the ones that come first. Yeah, so collaboration is the key. I have a question so like different synchrotron facilities, does LNS have PhD programs or exchange programs for postdocs that you would like to recognize in the audience. Thank you very much. Yes, indeed. We, we don't actually hold a PhD program. We typically have our scientists they can be PhD advisors to you through universities. We are currently not connected to any university this is a, as I said is a ministry facility. But we do have, for instance, agreements with some Carlos University and University of Campinas which is one of the largest in the country. And through these collaborations that the students can have their PhD done in the lab. Now we do have opportunities for. I think that we still have a project an ongoing project if you need more details, please email me, but an exchange program between Brazil and Sweden that allows scientists and students to travel from one facility to the other. I know that we have, we, some of our scientists, they went through to several of the bean lines to acquire experience from this first pioneer project. And that was very important for a facility as well. And I think this problem, this program of funding is still ongoing. I don't know unfortunately the details but obviously is halted by the pandemic. But I think that we still have one or two more years to come that will give opportunity for these exchanges. Excellent. So please, if you're interested have a look. Other questions. So thanks for the representation. Is it possible to get micro-HRF mapping from the nanometer based samples in any of these first installed bean lines? Yes, indeed. In fact, the Karnauba bean line that is in commissioning, the first experimental station that was installed, the result that showed from the soil is already a mapping based on micro-HRF fluorescence. The aim here is to go to 100 nanometer from this station, but it's a large working distance patient. So we, this basically the nano probing line has two major experimental stations, one based on 100 nanometers. And with large working distance so that we can put environments like electrochemical cells, or horizocosm, or batteries and different in situ systems. And it is already available for user commissioning. So it's one of the bean lines that we expect to have samples shipped from users as soon as we open for user commissioning. But it's already working with let's say sub-micrometer, micrometer and for X-ray fluorescence. Now the next experimental station is the challenging one. This is a cryo-nanoprobe that we aim to reach nanometer resolution, but also with the fraction and X-ray fluorescence. Okay. So someone in the audience wants to say that the Physics Institute at the University of Sao Paulo has an agreement with Uppsala University. So that's good to know. So another question maybe, great that there is a focus on data analysis modeling. Do you have data scientists to support the analysis? That was a decision, thanks for the question again. That was a decision we made in the early days of the project. We didn't have back in the project in the early days to 2013 when this project started. But we figured that the key aspect of a synchro in terms of what you can do is, and it's totally, let's say the major strength is 3D imaging. Of course we have several major strengths and synchro facilities, but in terms of resolution and imaging, 3D imaging because X-rays can penetrate matter. But we understood very soon that the amount of data and the algorithms that had to be developed, they were key for the success of the facility. So yes, we implemented a scientific computing group back then, which now basically is one of the groups that interfaces with the bean lines and with the user and demonstrates the high performance computer center and also the software tools. So they are the ones, they also work like administrator of a facility. So as users, they will interact with the scientific computing group that developed the algorithms from data processing all the way to data handling, which is something we've been dedicating a lot of effort with NVIDIA as well, as well as the tools for data visualization and data visualization. That's great. So we're running out of time. So I think we can ask you the last question. So I apologize with the audience for the other missed the question, but please feel free to contact or to reach out, maybe to speak with this and answer the next question. So last question. Last question, yes. So very nice results on the plant roots, but what's the effect of the X-rays on the plant? The X-ray energy was used because like you showed some results about studies on plants with X-rays. So the question is, what X-ray energy was used and what was the effect of the X-rays on the plant? Okay, got it. So the first, I think the first data I showed was from a high energy tomography beam line that's the root into the soil and that was with a filtered pink beam. So I think that the average energy was about 50 KV. This is the beam line. Let me tell the whole story in fact. The first beam line that we assembled was a pre-assembly of the X-ray tomography beam line. This beam line is going to work with 20, 40 and 70 KV. So when the project was starting to, when we were starting to get the first accumulated electron beam, then we figured that it would be important to pre-assemble this beam line even without optics so that we could take the first images of studios and show that it's actually working the whole pipeline, not only electron beam, but we could show 3D images. So we had this beam line working for almost a year until we get the optics. We had it working with a white filtered white beam. So basically it was a white beam from a superband of 3.2 Tesla, so the critical energy around 20 KV, but we filtered so to have the highest part, the highest energy part of the beam. I'm not sure what was the filtering that they were using, but this was a high energy tomography. Nevertheless, yes indeed we observe damage on the roots and this is probably the most critical part on the science, is how do you believe that especially what we want to see is roots growing into the soil. How do you believe that the radiation doesn't affect the soil and the growth of the root? Yes indeed, I think that a new version of the beam line now with monochromatic 70 KV will allow us to do that with much less damage. But yes, this is one of the main problems of working with living matter, but that's what science is about.