 This study evaluated the performance of three different antibody tests for detecting antibodies to the chlamydia trachomatis, CT, antigen PGP3. The tests included multiplex bead assay, MBA, enzyme-linked immunosorbent assay, ELISA, and lateral flow assay, LFA. The results showed that all three tests had high accuracy and precision when tested with two different clones of the chimeric antibody. Additionally, the stability of the clones was maintained over time when stored at minus 20°C and 4°C for up to two years. The limit of detection was similar for MBA and LFA, while ELISA was more than one lock fold lower, less sensitive. These findings suggest that the chimeric antibodies are suitable controls for testing antibodies to PGP3 and could help facilitate the deployment of these tests to other laboratories. This article was authored by Brook Goodhugh, Sailing Tang, Jason Goldstein, and others.