 After the completion of DNA extraction, we can confirm the presence of DNA through gel electrophoresis. So in this practical, we will demonstrate the examination of DNA through agarose gel electrophoresis. Basically, agarose gel electrophoresis is a process which is used to separate the DNA by size using the electric field that induces the negatively charged DNA molecules to migrate towards the positive pole through the matrix of agarose. Now, before proceeding to the experiment, we will look at the required materials and instruments. For the agarose gel electrophoresis, we need DNA template, 6x loading dye which is bromofenol blue, a thidium bromide which is a fluorescent dye used to visualize the DNA under UV light, agarose powder, 1x TAE buffer, pipettes, measuring cylinder, conical flask, gel caster, gel tank and electric supply. First of all, we will weigh the agarose powder with the help of weighing balance. We will weigh 0.8% to 1% agarose gel. For this purpose, we will weigh 1 gram of agarose and will add into 100 ml of TAE buffer. After weighing, agarose powder will be added to the conical flask. Next, measure the 1x TAE buffer in the cylinder and add into the flask. Mix the flask contents well and kept in oven for 1 minute. After 1 minute, take out the flask with the help of heat resistant glove and see whether contents have been dissolved or not. If contents are not dissolved, place the flask again in the oven until solution becomes clear. Let the flask cool for some extent. 7-8 microlitre a thidium bromide into the gel flask. It must be noted that thidium bromide should be handled carefully because it is carcinogenic dye. After adding the thidium bromide, now assemble the gel caster to pour the gel. Place comb in it to make wells. Now pour the gel in the caster carefully. When gel will be solidified, comb will be removed and gel will be placed in the tank. Add 1x TAE buffer into the gel. It should be noted that same buffer will be added into the gel tank that was used for the gel preparation. Add the buffer to the level that gel should be completely dissolved inside the buffer. Pour gel loading 2-3 microlitre of 6x loading dye. Pour to 5 microlitre of DNA template will be taken in PCR microfuge tubes. Mix the DNA with loading dye with the help of pipette and load the sample in the gel. The help of micro pipette mix the contents of loading dye and DNA template and then load into the gel. Similarly, all the DNA samples will be loaded in the gel. In first well, you will load the DNA ladder in order to quantify the size of DNA. After gel loading, cover the tank, plug in the electrode and turn on the power supply. Gel will be run at 90V for 30-35 minutes. When gel run will be completed, turn off the power supply and take the gel out of tank. We will visualize the gel in the gel dock. We will observe the gel UV light. We can confirm the DNA through the bands on the gel.