 This study investigated the antidiarrhea effect of aconite aqueous extract, AA, in mice and found that AA can reduce fecal water content and diarrhea scores, inhibit intestinal propulsion rate and pathological changes in the colon, increase AQP3 and AQP4 content in the colon, and regulate the gut microbiome. Additionally, AA was found to increase levels of taurine conjugated bile acids, TCA, dehydro, taurine conjugated bile acids, DCTA, lithicolic acid, LCA, dehydro, lithicolic acid, DLCA, and 12-ketolithicolic acid, 12-KLCA. These findings suggest that AA may have potential as a novel therapy for diarrhea. This article was authored by Dandan Zhang, Dandan Zhang, Hao Cheng, and others.