 Glutathione's physiological roles have expanded over the years, including nutrient metabolism, antioxidant defense, and regulation of cellular functions such as gene expression, DNA and protein synthesis, signal transduction, cell proliferation, and apoptosis. This review focuses on Glutathione's role in cell signaling by analyzing its ability to modulate nitroxidative stress, autophagy, and viral infection. This article was authored by Katia Iaquilano, Sarah E. Baldelli, and Maria Rosa E. Cerriolo.