 Gelatin has been widely used in pharmaceutical formulations, cell cultures, and tissue engineering due to its excellent biocompatibility, ease of processing, and low cost. In the past decade, Gelatin has been extensively investigated for various ocular applications such as cell sheet carriers, bio-adhesives, and bio-artificial grafts. These applications require distinct physical, chemical, and biological properties which has led to the development of modified Gelatin-based materials. Cross-linking Gelatin alone or in combination with natural or synthetic biopolymers has resulted in a variety of scaffolds that are suitable for ocular applications. This review discusses the methods used to cross-link Gelatin-based materials and their application in ocular tissue engineering. It also highlights future opportunities for Gelatin-based materials and ophthalmology. This article was offered by James B. Rose, Satimio Pacelli, Alicia J. L. Hodge and others. We are article.tv, links in the description below.