 Streptococcus pneumoniae contributes to a range of infections, including meningitis, pneumonia, otitis media, and sepsis. It has been suggested that infections by this bacterium may be associated with the phenomenon of molecular mimicry, which could lead to the induction of autoimmunity. To explore this hypothesis, we used a bioinformatics approach to identify 13S. Pneumoniae proteins that are similar to human proteins, with 11 of them being linked to autoimmune diseases like psoriasis, rheumatoid arthritis, and diabetes. We then used database mining to determine whether these proteins are involved in any known autoimmune disorders. Furthermore, we designed and validated an immune response via vaccine construction based on the antigenic, non-allergenic, and immunogenic sequences of these proteins. Our simulations and binding affinity assessments showed that Construct C8 binds to HLA molecules and TLR4 receptors with promising results. Additionally, our mapping of these mimics as conserved regions suggests. This article was authored by Mutabem Mushraki, Ahmad Al-Zamami, Nora Al-Turki, and others. We are article.tv, links in the description below.