 Our study showed that using a mixture of sheep manure and chemical fertilizer can lead to improved pair productivity by increasing the abundance of beneficial bacteria in the soil. Specifically, the ratio of sheep manure to chemical fertilizer had a positive effect on the growth of gamapretibacter and costridia, which are associated with AC, N ratio of 14 to 1 and the presence of key minerals such as potassium, iron, zinc, and copper. These minerals are essential for the transition from vegetative to reproductive growth, which leads to an increase in pair production. Additionally, blastociella was shown to act as a buffering system in regulating soil acidity, further improving the environment for plant growth. This article was authored by Chunhuisha, Xiaoching Wang, Chuang Jiang, and others.